Table 3 Affinities of ATG8 domains for the integrin β3 peptide.

KI values for ATG8 domains calculated from displacement experiments using unphosphorylated integrin β3 peptide and phosphorylated peptides (pThr777, pSer778, pThr779, pTyr785, or pThr779 pTyr785). Indicated error is the error of the mean (SEM); n = 3 replicates. N.B., no or low affinity (KI > 100 μM); N.M., not measured.

Peptide sequencesKI values ± SEM (μM)
MAP1LC3A
ATG8
MAP1LC3B
ATG8
MAP1LC3C
ATG8
GABARAPL2
ATG8
GABARAP
ATG8
Unphosphorylated
CH3CO-
KEATSTFTNITYRG-NH2
N.B.N.B.N.B.N.B.N.B.
pThr777
CH3CO-
KEApTSTFTNITYRG-NH2
N.B.N.B.73 ± 4N.M.47 ± 3
pSer778
CH3CO-
KEATpSTFTNITYRG-NH2
29.4 ± 0.5N.B.16.1 ± 0.580 ± 5N.M.
pThr779
CH3CO-
KEATSpTFTNITYRG-NH2
N.B.N.B.82 ± 3N.B.N.M.
pTyr785
CH3CO-
KEATSTFTNITpYRG-NH2
38 ± 281 ± 283 ± 489 ± 12N.M.
pThr779/pTyr785
CH3CO-
KEATSpTFTNITpYRG-NH2
8.5 ± 0.515 ± 18 ± 2N.M.17.8 ± 0.8