Table 2 Affinities of AP2 μ2, ATG8 domains of GABARAPs and MAP1LC3s, and NCK1 SH2 domains for probe peptides as determined by FP.

Equilibrium dissociation constant (KD) values of AP2 μ2, the ATG8 domains, and NCK1 SH2 for the respective FITC-labeled peptides (ATG9A, SQSTM1, and Tir10). Indicated errors are the errors of the mean (SEM); n = 3 replicates.

Peptide sequenceProtein domains
Protein domainKD ± SEM (μM)
ATG9A4–14
FITC-FDTEYQRLEAS-NH2
AP2 μ21.64 ± 0.07
SQSTM1335–344
FITC-DDDWTHLSSK-NH2
GABARAP ATG80.9 ± 0.1
GABARAPL2 ATG811 ± 1
MAP1LC3A ATG80.81 ± 0.04
MAP1LC3B ATG81.9 ± 0.1
MAP1LC3C ATG80.33 ± 0.01
Tir10371–280
FITC-EHIpYDEVAAD-NH2
NCK1 SH20.055 ± 0.006